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Guanylyl cyclase (soluble) (bovine lung)

 
ALX-202-039-C005 5 µg 298.00 USD
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Product Specification

Alternative Name:GTP pyrophosphate-lyase (cycling)
 
Source:Isolated from bovine lung by an immunoaffinity method.
 
EC:4.6.1.2
 
Concentration:

  

 
Formulation:Liquid. In ~20µl containing 50% glycerol, 88mM NaCl, 1mM DTT, 0.5mM EDTA, 25mM triethanolamine at pH 7.4.
 
Specific Activity:>10'000nmol cGMP/mg/min at 37°C under NO-stimulated conditions.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Product should be active for at least one year after shipping date when stored at -80°C under nitrogen in the presence of 50% glycerol. Dilutions of the enzyme should be peformed in the presence of 0.5mg/ml BSA. The dissolved product is stable for 8 months when stored in 50% glycerol at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

Detection of nitric oxide production in cell cultures by luciferin-luciferase chemiluminescence: Y. Woldman, et al.; Biochem. Biophys. Res. Commun. 465, 232 (2015), Application(s): Cell culture, Abstract;
Purification of soluble guanylyl cyclase from bovine lung by a new immunoaffinity chromatographic method: P. Humbert, et al.; Eur. J. Biochem. 190, 273 (1990), Abstract;

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