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eNOS (human), (recombinant)

 
ALX-201-853-0010 10 µg 386.00 USD
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Product Specification

Alternative Name:Nitric oxide synthase (endothelial), NOS III
 
MW:~135kDa/subunit; homodimer.
 
Source:Produced in Sf9 cells. Untagged human eNOS.
 
EC:1.14.13.39
 
UniProt ID:P29474
 
Formulation:Liquid. In 50 mM TRIS, pH 7.4, containing 0.1mM EDTA, 10% glycerol, 100mM NaCl, 0.1mM DTT, 10 µM tetrahydrobiopterin.
 
Purity:≥90% (SDS-PAGE, Western Blot)
 
Specific Activity:≥125 U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric oxide) per minute at 37°C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:eNOS is relatively unstable, and loses activity on ice within 30 minutes. Prepare aliquots and snap-freeze in liquid nitrogen until ready for use.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 

General Literature References

Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases: E. Butt, et al.; J. Biol. Chem. 275, 5179 (2000), Abstract; Full Text
The Oxyhemoglobin Assay: Feelisch, M., et al.; Meth. Nitric Oxide Research 455-476 (1996),

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