Product Details
| Alternative Name: | Nitric oxide synthase (endothelial), NOS III |
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| MW: | ~135kDa/subunit; homodimer. |
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| Source: | Produced in Sf9 insect cells. Untagged human eNOS. Produced in a baculovirus expression system. |
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| EC: | 1.14.13.39 |
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| UniProt ID: | P29474 |
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| Formulation: | Liquid. In 50 mM TRIS, pH 7.4, containing 0.1mM EDTA, 10% glycerol, 100mM NaCl, 0.1mM DTT, 10 µM tetrahydrobiopterin. |
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| Purity: | ≥90% (SDS-PAGE, Western Blot) |
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| Specific Activity: | ≥120 U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric oxide) per minute at 37°C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Use/Stability: | eNOS is relatively unstable, and loses activity on ice within 30 minutes. Prepare aliquots and snap-freeze in liquid nitrogen until ready for use. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Blockade on Lin28a Prevents Cognitive Impairment and Disruption of the Blood-Brain Barrier Induced by Chronic Cerebral Hypoperfusion: J. M. Lee, et al.; Biomedicines
10, 852 (2022),
Abstract;
Protein kinase N2 mediates flow-induced eNOS activation and vascular tone regulation: Y.J. Jin, et al.; J. Clin. Invest.
9, 145734 (2021),
Abstract;
General Literature References
Endothelial nitric-oxide synthase (type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases: E. Butt, et al.; J. Biol. Chem.
275, 5179 (2000),
Abstract;
Full Text
The Oxyhemoglobin Assay: Feelisch, M., et al.; Meth. Nitric Oxide Research 455-476 (1996),
