| Alternative Name: | FGF2, HBGF-2, Prostatropin |
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| MW: | ~16.3kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 145 amino acids. |
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| UniProt ID: | p15655 |
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| Formulation: | Lyophilized from 5mM Na2PO4, pH 7.5 and 50mM sodium chloride. Sterile filtered. |
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| Purity: | ≥95% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent proliferation of BALB/3T3 cells which is typically observed at concentations <1ng/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Fibroblast growth factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic, or FGF-1, is a critical component in keeping human embryonic stem cells undifferentiated in cell culture systems. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse FGF-basic has a predicted MW of 17.2 kDa.
Figure 1: Mouse FGF basic Bioactivity Data. Serial dilutions of mouse FGF Basic, starting at 5ng/ml, were added to NIH 3T3 cells. Cell proliferation was measured after 41 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse FGF Basic was 0.1-0.16ng/ml. This value is comparable with the typical expected range of < 1ng/ml.
