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FGF-basic 154 (human), (recombinant)

 
ALX-201-823-0010 10 µg 138.00 USD
 
ALX-201-823-0050 50 µg 205.00 USD
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  • Carrier-free

Product Specification

Alternative Name:FGF2, HBGF-2, Prostatropin
 
MW:~17.2kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 154 amino acids.
 
UniProt ID:P09038 + 7 aa of propeptide
 
Formulation:Lyophilized from 10mM sodium phosphate, 75mM NaCl, pH 7.5. Sterile filtered.
 
Purity:≥97% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <1ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Fibroblast growth factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 154, also known as FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
 
ALX-201-823 Fig1
Figure 1: Human FGF-basic 154 Bioactivity Data. Serial dilutions of human FGF Basic 154, starting at 5ng/ml, were added to NIH 3T3 cells. Cell proliferation was measured after 41 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of human FGF Basic was 0.1-0.14ng/ml. This value is comparable with the typical expected range of < 1ng/ml.
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ALX-201-823 Fig1

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