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FGF-basic 147 (human), (recombinant)

 
ALX-201-822-0010 10 µg 138.00 USD
 
ALX-201-822-0050 50 µg 230.00 USD
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  • Carrier-free

Product Specification

Alternative Name:FGF2, HBGF-2, Prostatropin
 
MW:~16.5kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 147 amino acids.
 
UniProt ID:P09038
 
Formulation:Lyophilized from 10mM Na2PO4, pH 8.0. Sterile filtered.
 
Purity:≥95% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <1ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Fibroblast growth factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154), also called FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems.
 
ALX-201-822 Fig1
Figure 1: Human FGF-basic 147 Bioactivity Data. 3T3 cells were cultured with 0 to 10ng/ml human FGF basic. Cell proliferation was measured after 44 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of human FGF basic was 0.042-0.063ng/ml. The typical expected range is less than 1ng/ml.
ALX-201-822 Fig2
Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human FGF basic has a predicted MW of 16.5 kDa.
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ALX-201-822 Fig1 ALX-201-822 Fig2

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