| Alternative Name: | Interleukin-3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor |
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| MW: | ~15kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 134 amino acids. |
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| UniProt ID: | P08700 |
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| Formulation: | Lyophilized. Sterile filtered. |
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| Purity: | ≥95% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent stimulation of TF-1 cells which is typically observed at concentations ED50 ≤2 ng/mL. |
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| Reconstitution: | Reconstitute in sterile water at 0.1 mg/mL. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases. |
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| Regulatory Status: | RUO - Research Use Only |
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