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IL-3 (human), (recombinant)

 
ALX-201-820-0010 10 µg 218.00 USD
 
ALX-201-820-0100 100 µg 780.00 USD
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  • Carrier-free

Product Specification

Alternative Name:Interleukin-3, MCGF, Multi-CSF, HCGF, P-cell stimulation factor
 
MW:~15kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 134 amino acids.
 
UniProt ID:P08700
 
Formulation:Lyophilized. Sterile filtered.
 
Purity:≥95% (Reducing and Non-reducing SDS-PAGE)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent stimulation of TF-1 cells which is typically observed at concentations ED50 ≤2 ng/mL.
 
Reconstitution:Reconstitute in sterile water at 0.1 mg/mL.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Interleukin-3 (IL-3) is a cytokine produced by activated T cells and mast cells. IL-3 is able to induce the differentiation of hematopoietic stem cells to precursor cells of myeloid lineage (erythrocytes, megakaryocytes, granulocytes, dendritic cells and monocytes). IL-3 also has functions in the nervous system and appears to be important in several chronic inflammatory diseases.
 
ALX-201-820 Fig1
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human IL-3 has a predicted MW of 15 kDa.
ALX-201-820
Figure 2: TF-1 cells were cultured with 0 to 100 ng/mL Human IL-3. Cell proliferation was measured after 72 hours and the linear portion of the curve can be used to calculate the ED50.
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ALX-201-820 Fig1 ALX-201-820

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