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IL-6 (mouse), (recombinant)

 
ALX-201-819-0010 10 µg 205.00 USD
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  • Carrier-free

Product Specification

Alternative Name:Interleukin-6, IFN-β2, B-cell differentiation factor, BSF-2, HSF, MGI-2
 
MW:~21.7kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 187 amino acids.
 
UniProt ID:P08505
 
Formulation:Lyophilized. Sterile filtered.
 
Purity:≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by dose-dependent induction of 7TD1 cell proliferation which is typically observed at concentations <0.02ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized mouse IL-6 in 10 mM HCl to 0.1-1.0 mg/ml, which can be further diluted to other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells.
 
ALX-201-819 Fig1
Figure 1: Mouse IL-6 Bioactivity Data. 7TD1 cells were cultured with 0 to 250pg/ml mouse IL-6. Cell proliferation was measured after 66 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse IL-6 was 2.4-3.6pg/ml. This value is comparable to expected range of less than 20pg/ml.
ALX-201-819 Fig2
Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse IL-6 has a predicted MW of 21.7 kDa.
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ALX-201-819 Fig1 ALX-201-819 Fig2

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