Product Details
| Alternative Name: | Interleukin-6, IFN-β2, B-cell differentiation factor, BSF-2, HSF, MGI-2 |
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| MW: | ~21kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 184 amino acids. |
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| UniProt ID: | P05231 |
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| Formulation: | Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10mM sodium phosphate, pH 7.5. |
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| Purity: | ≥95% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/μg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | B9 Cell Proliferation ED50 ≤25 pg/mL (≥4.0 x 10^7 units/mg) |
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| Reconstitution: | Sterile water at 0.1 mg/mL |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Add 0.1% BSA for long term storage. |
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| Handling: | Store at -80°C after reconstitution |
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| Scientific Background: | Interleukin 6 (IL-6) is an important pro-inflammatory and anti-inflammatory cytokine expressed by T cells, macrophages and muscle cells. IL-6 signals through a receptor complex containing two receptors, IL-6Rα and gp130. IL-6 has an important function in promoting fever and can serve to stimulate an immune response to trauma. IL-6 is often used for growth of hybridoma cell lines. Human IL-6 is active on mouse and rat cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human IL-6 has a predicted MW of 20.9 kDa.
Figure 2: B9 cells were cultured with dilutions of Human IL-6. Cell proliferation can be measured and the linear portion of the curve can be used to calculate the ED50.
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Product Literature References
Crystallins Play a Crucial Role in Glaucoma and Promote Neuronal Cell Survival in an In Vitro Model Through Modulating Müller Cell Secretion: H. Liu, et al.; Invest. Ophthalmol. Vis. Sci.
63, 3 (2022),
Abstract;
TLR5 activation by flagellin induces doxorubicin resistance via interleukin-6 (IL-6) expression in two multiple myeloma cells: H.Y. Cho, et al.; Cell. Immunol.
289, 27 (2014),
Abstract;
