| Alternative Name: | FS, Activin-binding protein, FSH-suppressing protein |
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| MW: | ~31.6kDa |
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| Source: | Produced in E. coli. Non-glycosylated protein, containing 289 amino acids. |
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| UniProt ID: | P47931 |
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| Formulation: | Lyophilized from 10mM Na2PO4, pH 7.5, containing 50mM sodium chloride. Sterile filtered. |
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| Purity: | ≥90% (Reducing and Non-reducing SDS-PAGE) |
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| Endotoxin Content: | ≤5 EU/µg protein measured by kinetic LAL analysis. |
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| Appearance: | White lyophilized (freeze-dried) powder. |
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| Biological Activity: | The activity is determined by the dose-dependent neutralization of 7.5ng/ml human Activin A. Complete neutralization is typically reached at <0.3µg/ml. |
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| Reconstitution: | When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. |
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| Handling: | Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. |
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| Scientific Background: | Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-β family members (activin, BMPs, myostatin, GDF-11 and TGF-β1). Mechanistically, follistatin works as an antagonist by complexing with TGF-β family members to prevent them from interacting with their signaling receptors. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure 1: Mouse Follistatin Bioactivity Data. Serial dilutions of mouse Follistatin (starting at 1µg/ml) were mixed with 7.5ng/ml human Activin A, then added to MCP-11 cells. After 69 hours cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse Follistatin was between 0.13-0.19µg/ml. This value is comparable to the typically expected to be less than 0.3µg/ml.
Figure 2: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Mouse Follistatin has a predicted MW of 31.6 kDa.
