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Epidermal growth factor (rat), (recombinant)

 
ALX-201-812-0100 100 µg 230.00 USD
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  • Carrier-free

Product Specification

Alternative Name:EGF, Urogastrone, URG
 
MW:~6.3kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 54 amino acids, with a molecular weight of 6.3 kDa.
 
UniProt ID:P07522
 
Formulation:Lyophilized from 10mM Na2PO4, pH 7.5. Sterile filtered.
 
Purity:≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <0.1ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4.
 
ALX-201-812 Fig1
Figure 1: Rat EGF Bioactivity Data. 3T3 cells were cultured with 0 to 1ng/ml Rat EGF. Cell proliferation was measured after 42 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of Rat EGF was 50-80pg/ml. This value is comparable to the typical expected range of 20-100pg/ml.
ALX-201-812 Fig2
Figure 2: 1µg of protein run under (+) reducing and (-) non-reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Rat EGF is predicted to have a MW of 6.3 kDa.
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ALX-201-812 Fig1 ALX-201-812 Fig2

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