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Stem cell factor (human), (recombinant)

 
ALX-201-804-0002 2 µg 138.00 USD
 
ALX-201-804-0010 10 µg 230.00 USD
Do you need bulk/larger quantities?
 
  • High purity
  • Carrier-free

Product Specification

Alternative Name:SCF, c-Kit ligand, KL, Steel factor, MGF
 
MW:~18.4kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 165 amino acids.
 
UniProt ID:P21583
 
Formulation:Lyophilized from 10mM acetic acid (AcOH) at a concentration of 1mg/ml. Sterile filtered.
 
Purity:≥98% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent stimulation of human TF-1 cells which is typically observed at a concentration of 1-5ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.
 
ALX-201-804 Fig1
Figure 1: Human SCF Bioactivity Data. Serial dilutions of human SCF, starting at 250ng/ml, were added to TF-1 cells. After 68 hours, cell proliferation was measured and the linear portion of the curve was us used to calculate the ED50. The ED50 for the lot shown of human SCF is 3.3-5ng/ml. This value is comparable to the typical expected value of 1-5ng/ml.
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ALX-201-804 Fig1

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ALX-201-805-0002 2 µg 138.00 USD
 
ALX-201-805-0010 10 µg 214.00 USD
Do you need bulk/larger quantities?
 

Stem cell factor (rat), (recombinant) 

Produced in E. coli. Non-glycosylated protein, containing 165 amino acids., ≥98% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) | Print as PDF
 
ALX-201-806-0002 2 µg 138.00 USD
 
ALX-201-806-0010 10 µg 230.00 USD
Do you need bulk/larger quantities?
 

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