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Flt-3 ligand (human), (recombinant)

 
ALX-201-800-0002 2 µg 138.00 USD
 
ALX-201-800-0010 10 µg 230.00 USD
Do you need bulk/larger quantities?
 
  • Carrier-free

Product Specification

Alternative Name:FMS-related tyrosine kinase 3 ligand
 
MW:~17.6kDa
 
Source:Produced in E. coli. Non-glycosylated protein, containing 155 amino acids.
 
UniProt ID:P49771
 
Formulation:Lyophilized from sterile filtered solution containing 0.1% TFA.
 
Purity:≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
 
Endotoxin Content:≤1 EU/μg protein measured by kinetic LAL analysis.
 
Appearance:White lyophilized (freeze-dried) powder.
 
Biological Activity:The activity is determined by the dose-dependent stimulation of the proliferation of human Aml5 cells which is typically observed at a concentration of 0.6-1.0ng/ml.
 
Reconstitution:When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
 
Handling:Centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:FMS-related tyrosine kinase 3 ligand (Flt-3 ligand) is a growth factor important for the proliferation of hematopoietic cells. Flt-3 ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like tyrosine kinase-3 (Flt-3). Flt-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt-3 ligand can stimulate the proliferation of cells expressing mouse Flt-3 receptors.
 
ALX-201-800 Fig1
Figure 1: 1µg in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Human Flt-3 ligand has a predicted MW of 17.6 kDa.
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ALX-201-800 Fig1

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