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DLL1 (soluble) (human), (recombinant)

Notch ligand
 
ALX-201-765-0025 25 µg 359.00 USD
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Product Specification

Alternative Name:Delta-like protein 1, Delta1
 
MW:~57-60kDa
 
Source:Produced in HEK 293 cells. Recombinant human sDLL-1 contains 522 amino-acid residues.
 
UniProt ID:O00548
 
GenBank ID:28514
 
Formulation:Lyophilized from 1X PBS, pH 7.5.
 
Purity:≥95% (SDS-PAGE and HPLC)
 
Endotoxin Content:< 0.1 ng per μg of sDLL-1.
 
Activity:Inhibits adipogenesis of mesenchymal stem cells.
 
Biological Activity:Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3-5 μg/ml.
 
Reconstitution:Centrifuge the vial prior to opening. Reconstitute in water at a concentration of 0.1 - 1mg/ml. Do not vortex. For long term storage, it is recommended to dilute further in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted sDLL-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
 
Scientific Background:Notch signalling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands are divided into two subclasses, the delta and the serrate family. Delta-like 1, 3, and 4 (DLL1, -3, -4), JAG1 and JAG2 with DSL (Delta, Serrate, Lag2) domain are typical Notch ligands, while DNER, F3/Contactin and NB-3 without DSL domain are atypical Notch ligands. Notch-ligand binding to NOTCH1, -2, -3, or -4 receptor induces the receptor proteolysis by metalloprotease and γ-secretase to release Notch intracellular domain (NICD).
Human sDLL-1 comprises the extracellular signaling domain of DLL1, a member of a structurally-related family of single-pass type I trans-membrane proteins that serve as ligands for Notch receptors. It is expressed in the heart and pancreas, and to a lesser extent in various other tissues. DLL-1 functions to specifically activate the Notch-1 and Notch-2 receptors. The Notch signaling pathway regulates endothelial-cell differentiation, proliferation and apoptosis, and is essential for the development, maintenance and remodeling of the vascular system. DLL-1 suppresses differentiation of hematopoietic progenitor cells into the B-cell lineage while promoting differentiation to T-cell and NK cell precursors.
 

Product Literature References

Actin cytoskeleton regulator Arp2/3 complex is required for DLL1 activating Notch1 signaling to maintain the stem cell phenotype of glioma initiating cells: C. Zhang, et al.; Oncotarget 8, 33353 (2017), Abstract; Full Text

General Literature References

Notch signaling in gastrointestinal tract: M. Katoh; Int. J. Oncol. 30, 247 (2007), Review, Abstract;
Proteolytic processing of delta-like 1 by ADAM proteases: E. Dyczynska, et al.; J. Biol. Chem. 282, 436 (2007), Abstract; Full Text
The Notch Ligand Delta-Like 1 is Essential for Postnatal Arteriogenesis: A. Limbourg, et al.; Circ. Res. 100, 363 (2007), Abstract;
NOTCH signaling as a novel cancer therapeutic target: L. Miele, et al.; Curr. Cancer Drug Targets 6, 313 (2006), Abstract;
Notch-induced proteolysis and nuclear localization of the Delta ligand: C.E. Bland, et al.; J. Biol. Chem. 278, 13607 (2003), Abstract; Full Text
The Notch ligand Delta1 is sequentially cleaved by an ADAM protease and gamma-secretase: E. Six, et al.; PNAS 100, 7638 (2003), Abstract; Full Text
The Notch ligands, Delta1 and Jagged2, are substrates for presenilin-dependent "gamma-secretase" cleavage: T. Ikeuchi and S.S. Sisodia; J. Biol. Chem. 278, 7751 (2003), Abstract; Full Text
The Notch ligands, Jagged and Delta, are sequentially processed by alpha-secretase and presenilin/gamma-secretase and release signaling fragments: M.J. LaVoie and D.J. Selkoe; J. Biol. Chem. 278, 34427 (2003), Abstract; Full Text
Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation: A.C. Jaleco, et al.; J. Exp. Med. 194, 991 (2001), Abstract; Full Text
A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells: W. Han, et al.; Blood 95, 1616 (2000), Abstract; Full Text
Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999), Abstract; Full Text
Transient and restricted expression during mouse embryogenesis of Dll1, a murine gene closely related to Drosophila Delta: B. Bettenhausen, et al.; Development 121, 2407 (1995), Abstract; Full Text
The molecular genetics of early neurogenesis in Drosophila melanogaster: E. Knust and J.A. Campos-Ortega; Bioessays 11, 95 (1989), Abstract;

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