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Highly active serine protease providing superior results.
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Potent negative regulator of hepcidin.
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Eukaryotic cell-derived protein guarantees proper PTM.
Product Details
| Alternative Name: | Transmembrane protease serine 6, TMPRSS6 |
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| MW: | ~84kDa; a-subunit: ~56kDa; b-subunit: ~28kDa |
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| Source: | Produced in Drosophila Schneider 2 (S2) cells. Recombinant human matriptase-2 (aa 78-811) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression. |
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| EC: | 3.4.21.109 |
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| UniProt ID: | Q8IU80 |
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| Formulation: | Liquid. In 100mM TRIS-HCl, pH 9.0, containing 150 mM sodium chloride and 20% glycerol. |
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| Purity Detail: | Purified by multi-step chromatography. |
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| Activity: | ≥2.5 U/µl
Please see Certificate of Analysis for detailed activity information. |
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| Biological Activity: | Trypsin-like serine protease. |
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| Specific Activity: | One U = 10 pmol/min, using Boc-Gln-Ala-Arg-AMC (50µM, Prod. No. BML-P237) as substrate at 37°C. |
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| Shipping: | Dry Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Use/Stability: | Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 0.5mg/ml BSA. Do not store diluted solutions. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | Matriptase-2 (TMPRSS6) is a type II transmembrane serine protease mainly produced by the liver. It acts as a potent negative regulator of hepcidin, a hepatic peptide hormone and integral player in regulating iron homeostasis.
Matriptase-2 plays an important role in understanding iron disorders; including various anemias and iron overload conditions, affecting millions of people worldwide. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
A New Method to Assay Protease Based on Amyloid Misfolding: Application to Prostate Cancer Diagnosis Using a Panel of Proteases Biomarkers: H. Li, et al.; Theranostics
4, 701 (2014),
Application(s): Assay,
Abstract;
Full Text
Identification of the first low-molecular-weight inhibitors of matriptase-2: M.T. Sisay, et al.; J. Med. Chem.
53, 5523 (2010),
Abstract;
Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J.
276, 2213 (2009),
Abstract;
General Literature References
Matriptase-2- and proprotein convertase-cleaved forms of hemojuvelin have different roles in the down-regulation of hepcidin expression: J.E. Maxson, et al.; J. Biol. Chem.
285, 39021 (2010),
Abstract;
Membrane-bound serine protease matriptase-2 (Tmprss6) is an essential regulator of iron homeostasis: A.R. Folgueras, et al.; Blood
112, 2539 (2008),
Abstract;
Matriptase-2 inhibits breast tumor growth and invasion and correlates with favorable prognosis for breast cancer patients: C. Parr, et al.; Clin. Cancer Res.
13, 3568 (2007),
Abstract;
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