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Hepsin (human), (recombinant)

 
ALX-201-750-1 125 U 348.00 USD
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Product Specification

Alternative Name:Transmembrane protease serine 1, TMPRSS1
 
MW:~45kDa; a-subunit: ~15kDa; b-subunit: ~30kDa
 
Source:Produced in Drosophila Schneider 2 (S2) cells. Recombinant human hepsin (aa 45-417) is fused at the C-terminus to a V5-His-tag.
 
EC:3.4.21.106
 
UniProt ID:P05981
 
Concentration:100nM
 
Formulation:Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride and 10% glycerol.
 
Biological Activity:Trypsin-like serine protease.
 
Specific Activity:~2.5U/µl. One unit is defined at the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50µM Boc-Gln-Ala-Arg-AMC.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 5mg/ml BSA. Do not store diluted solutions.
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J. 276, 2213 (2009), Abstract;

General Literature References

HEPSIN inhibits cell growth/invasion in prostate cancer cells: V. Srikantan, et al.; Cancer Res. 62, 6812 (2002), Abstract;

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