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IDO (human), (recombinant) (His-tag)

 
ALX-201-501-0050 50 µg 514.00 USD
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Product Specification

Alternative Name:Indoleamine 2,3-dioxygenase, INDO
 
Source:Produced in E. coli. Mature human IDO (indoleamine 2,3-dioxygenase) (aa 1-403) is fused at the C-terminus to a His-tag.
 
EC:1.13.11.52
 
UniProt ID:P14902
 
Formulation:Liquid. 0.2µm-filtered solution in 50mM TRIS, pH 7.5, containing 100mM NaCl.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:<1.0EU/µg protein (LAL test; Bio Whittaker).
 
Specific Activity:> 200 U/μg.  One unit is defined as the conversion  of 1 pmol/min L-tryptophan to N-formylkynurenine.
 
Applications:WB
Activity assay
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Working aliquots are stable for up to 12 months when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:Indoleamine 2,3-dioxygenase (IDO) is an enzyme that catalyzes the degradation of L-tryptophan to N-formylkynurenine. This immunomodulatory enzyme has been shown to allow tumor cells to escape the immune system by depletion of L-Trp in the microenvironment of cell A wide range of human cancers overexpress human IDO (hIDO).
 
ALX-201-501 SDS-PAGE
SDS-PAGE analysis - Lane 1: MW Marker, Lane 2: 1 μg IDO. (Prod. No. ALX-201-501, IDO (human), (recombinant) (His-tag))
ALX-201-501 WB
Western blot analysis – Lane 1: MW Marker, Lane 2: 100 ng IDO. Probed with pAb raised against full-length human recombinant IDO. (Prod. No. ALX-201-501, IDO (human), (recombinant) (His-tag)).
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ALX-201-501 SDS-PAGE ALX-201-501 WB

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