Pro-IL-33 (human), (recombinant) (His-tag)



ALX-201-471-C010	10 µg	382.00 USD

Product Specification

Alternative Name:	Interleukin-33, NF-HEV, IL-1F11

Concentration:	0.25mg/ml

Purity Detail:	Purified using Ni-NTA column and FPLC.

Endotoxin Content:	<1.0EU/µg purified protein (LAL test)

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in 55mM TRIS-HCl, pH 8.2, containing 150mM sodium chloride.

Source/Host:	Produced in E. coli. The mature domain of recombinant human IL-33 (aa 1-270) is fused at the C-terminus to a His-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Background / Technical Information:	UniProt ID O95760: IL-33 (human)

Figure 1. NH2-terminal sequence analysis of Pro-IL-33 (human), (recombinant) (His-tag) (Prod. No. ALX-201-471).

Figure 2. Pull down assay - pull down of Pro-IL-33-His (human) by ST2-Fc (human)
Method 5µg of human ST2-Fc, 2µg of each of human pro-IL-33, human IL-33 and human IL-37 (control) and protein A resin were incubated in 0.5ml RIPA buffer overnight at 4°C. The precipitates were separated by SDS-PAGE, electro-transfered onto NC membrane and subjected to immunoblotting with IL-33 (human), mAb (IL3335B).

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General Literature References

The IL-1-like cytokine IL-33 is inactivated after maturation by caspase-1: C. Cayrol & J.P. Girard; Proc. Nat.l Acad. Sci. USA 106, 9021 (2009), Abstract; Full Text

IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo: V. Carriere et al.; Proc. Natl. Acad. Sci. USA 104, 282 (2007), Abstract;

IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005), Abstract;

Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules: E.S. Baekkevold, et al. ; Am. J. Pathol. 163, 69 (2003), Abstract;