DLL1 (mouse):Fc (human), (recombinant)

Notch ligand



ALX-201-455-C010	10 µg	185.00 USD

ALX-201-455-C050	50 µg	382.00 USD

Interacts with Notch receptors.

Product Specification

Alternative Name:	Delta-like Protein 1

Concentration:	0.2mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant mouse DLL1 (Delta-like protein 1) (aa 1-545) is fused at the C-terminus to the Fc portion of human IgG.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Notch signaling regulates cell fate decisions during development and in the adult. The signaling pathway is activated by direct interactions between the Notch receptor and a DSL (Delta/Serrate/Lag2) ligand. In mammals, there are four different Notch receptors (Notch 1-4), and five DSL ligands (Delta-like 1,3, and 4 and Jagged 1 and 2).

Background / Technical Information:	UniProt ID Q61483: DLL1 (mouse)

Figure 1: SDS-PAGE showing DLL1 (mouse):Fc (human) (recombinant) (Prod. No. ALX-201-455).

Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DLL1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-455).
Method: 3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1μM dexamethasone, 0.5mM IBMX, 10μg/ml insulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-α(20ng/ml) was added. Recombinant mouse DLL1-Fc (5ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells. Visualized at three different spots.

Figure 3: Induction of Hes-1 with the treatment of DLL1 (mouse):Fc (human) (recombinant) (Prod. No. ALX-201-455).Method: A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-mouse Hes1 or GAPDH.

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General Literature References

The Notch ligands, Delta1 and Jagged2, are substrates for presenilin-dependent "gamma-secretase" cleavage: T. Ikeuchi and S.S. Sisodia; J. Biol. Chem. 278, 7751 (2003), Abstract; Full Text

Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation: A.C. Jaleco, et al.; J. Exp. Med. 194, 991 (2001), Abstract; Full Text

A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells: W. Han, et al.; Blood 95, 1616 (2000), Abstract; Full Text

Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999), Abstract; Full Text