Complement C1q tumor necrosis factor-related protein 5, C1qTNF5
Source:
Produced in E. coli. The mature peptide of human CTRP5 (aa 16-243) is fused at the N-terminus to a His-tag.
UniProt ID:
Q9BXJ0
Formulation:
Lyophilized from a 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM sodium chloride.
Purity:
≥90% (SDS-PAGE)
Endotoxin Content:
<1.0EU/µg purified protein (LAL test)
Reconstitution:
Reconstitute in an appropriate buffer.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Use/Stability:
Working aliquots are stable for up to 3 months when stored at -20°C.
Handling:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Scientific Background:
CTRP5 is a member of the CTRP familly designated as C1q tumor necrosis factor-related proteins. The CTRPs are paralogs of the adiponectin protein characterized by an N-termius variable region and C-terminal globular domain (C1q). CTRP5 is a putative secreted protein. A mutation in CTRP5 gene has been genetically linked to the Late-onset retinal degeneration (L-ORD) disease wich lead to severe visual loss.
Figure 2: CTRP5 (human) (rec.) (His) (Prod. No. ALX-201-451) activates AMPK signaling pathway in rat L6 myoblasts. Method: Differentiated rat L6 myoblasts were stimulated with control buffer or 0.5ug/ml CTRP5 (human) (rec.) (His) for 30min. The cell lysate was subjected to immunoblotting with antibodies against pAMPK and total AMPK.
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General Literature References
A family of Acrp30/adiponectin structural and functional paralogs: G.W. Wong, et al.; PNAS 101, 10302 (2004), Abstract;
Mutation in a short-chain collagen gene, CTRP5, results in extracellular deposit formation in late-onset retinal degeneration: a genetic model for age-related macular degeneration: C. Hayward, et al.; Hum. Mol. Genet. 12, 2657 (2003), Abstract;
Produced in E. coli. Recombinant mouse CTRP5 (Complement C1q tumor necrosis factor-related protein 5) (aa 16-243) is fused at the N-terminus to a linker peptide (14 aa) and a FLAG®-tag., ≥95% (SDS-PAGE) | Print as PDF
Produced in HEK 293 cells. The globular domain of mouse CTRP2 (C1q tumor necrosis factor-α-related protein 2) (aa 151-294) is fused at the N-terminus to a FLAG®-tag., ≥90% (SDS-PAGE) | Print as PDF
