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CTRP5 (human), (recombinant) (His-tag)

 
ALX-201-451-C010 10 µg 250.00 USD
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Product Specification

Alternative Name:Complement C1q tumor necrosis factor-related protein 5, C1qTNF5
 
Source:Produced in E. coli. The mature peptide of human CTRP5 (aa 16-243) is fused at the N-terminus to a His-tag.
 
UniProt ID:Q9BXJ0
 
Formulation:Lyophilized from a 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM sodium chloride.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<1.0EU/µg purified protein (LAL test)
 
Reconstitution:Reconstitute in an appropriate buffer.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Working aliquots are stable for up to 3 months when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:CTRP5 is a member of the CTRP familly designated as C1q tumor necrosis factor-related proteins. The CTRPs are paralogs of the adiponectin protein characterized by an N-termius variable region and C-terminal globular domain (C1q). CTRP5 is a putative secreted protein. A mutation in CTRP5 gene has been genetically linked to the Late-onset retinal degeneration (L-ORD) disease wich lead to severe visual loss.
 
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Figure 1: SDS-PAGE of CTRP5 (human) (rec.) (His) (Prod. No. ALX-201-451).
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Figure 2: CTRP5 (human) (rec.) (His) (Prod. No. ALX-201-451) activates AMPK  signaling pathway in rat L6 myoblasts.  Method: Differentiated rat L6 myoblasts were stimulated with control buffer or 0.5ug/ml CTRP5 (human) (rec.) (His) for 30min. The cell lysate was subjected to immunoblotting with antibodies against pAMPK and total AMPK.
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General Literature References

A family of Acrp30/adiponectin structural and functional paralogs: G.W. Wong, et al.; PNAS 101, 10302 (2004), Abstract;
Mutation in a short-chain collagen gene, CTRP5, results in extracellular deposit formation in late-onset retinal degeneration: a genetic model for age-related macular degeneration: C. Hayward, et al.; Hum. Mol. Genet. 12, 2657 (2003), Abstract;

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