CTRP5 (globular domain) (human), (recombinant) (His-tag)



ALX-201-443-C010	10 µg	231.00 USD

ALX-201-443-C050	50 µg	693.00 USD

Product Specification

Alternative Name:	Complement C1q tumor necrosis factor-related protein 5, C1qTNF5

Concentration:	~1mg/ml

Purity Detail:	Purified using Ni-NTA column and FPLC.

Endotoxin Content:	<1EU/µg purified protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2μm-filtered solution in 55mM Tris-Cl, pH 8.2, containing 150mM sodium chloride.

Source/Host:	Produced in E. coli. The globular domain of human CTRP5 (aa 96-243) is fused at the N-terminus to a His-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	CTRP5 is a member of the CTRP familly designated as C1q tumor necrosis factor-related proteins. The CTRPs are paralogs of the adiponectin protein characterized by an N-termius variable region and C-terminal globular domain (C1q). CTRP5 is a putative secreted protein. A mutation in CTRP5 gene has been genetically linked to the Late-onset retinal degeneration (L-ORD) disease wich lead to severe visual loss.

Background / Technical Information:	UniProt ID Q9BXJ0: Complement C1q tumor necrosis factor-related protein 5 (human)

Figure: SDS-PAGE of CTRP5 (Globular Domain) (human) (rec.) (His) (Prod. No. ALX-201-443).

Figure 2: CTRP5 (Globular Domain) (human) (rec.) (His) (Prod. No. ALX-201-443) activates AMPK signaling pathway in rat L6 myoblasts. Method: Differentiated rat L6 myoblasts were stimulated with control buffer or 0.5ug/ml CTRP5 (Globular Domain) (human) (rec.) (His) for 30min. The cell lysate was subjected to immunoblotting with antibodies against pAMPK and total AMPK.

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General Literature References

A family of Acrp30/adiponectin structural and functional paralogs: G.W. Wong, et al.; PNAS 101, 10302 (2004), Abstract;

Mutation in a short-chain collagen gene, CTRP5, results in extracellular deposit formation in late-onset retinal degeneration: a genetic model for age-related macular degeneration: C. Hayward, et al.; Hum. Mol. Genet. 12, 2657 (2003), Abstract;