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DNER (extracellular domain) (human):Fc (human), (recombinant)

Notch ligand
ALX-201-428-C010 10 µg 245.00 USD
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Product Specification

Alternative Name:BET, UNQ262/PRO299, Delta and Notch-like epidermal growth factor-related receptor
Source:Produced in HEK 293 cells. Signal peptide and extracellular domain of human DNER (aa 1-637) are fused at the C-terminus to the Fc portion of human IgG.
UniProt ID:Q8NFT8
Formulation:Liquid. 0.2µm-filtered solution in PBS.
Purity:≥90% (SDS-PAGE)
Endotoxin Content:<1EU/µg protein (LAL-test).
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Use/Stability:Working aliquots are stable for up to 3 months when stored at -20°C.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Figure 1: SDS-PAGE of DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428).
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Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For initiation of differentiation, 3T3L-1 cells were cultured in growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX and 10μg/ml insulin (day 0). The Medium was changed every 2 days and staining with Oil Red O was typically performed on day 7. As negative control, human TNF-α (20ng/ml) was used. To immobilize Notch ligands in the culture plates, plates were incubated with a solution of DNER (Extracellular Domain) (human):Fc (human) (rec.) (5μg/ml) or hCD137(4-1BB)-Fc (5μg/ml). Visualized at three different spots.
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