DNER (extracellular domain) (human):Fc (human), (recombinant)

Notch ligand



ALX-201-428-C010	10 µg	231.00 USD

ALX-201-428-C050	50 µg	693.00 USD

Product Specification

Alternative Name:	BET, UNQ262/PRO299, Delta and Notch-like epidermal growth factor-related receptor

Concentration:	0.5mg/ml

Endotoxin Content:	<1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of human DNER (aa 1-637) are fused at the C-terminus to the Fc portion of human IgG.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q8NFT8: DNER (human)

Figure 1: SDS-PAGE of DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428).

Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DNER (Extracellular Domain) (human):Fc (human) (rec.) (Prod. No. ALX-201-428).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For initiation of differentiation, 3T3L-1 cells were cultured in growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX and 10μg/ml insulin (day 0). The Medium was changed every 2 days and staining with Oil Red O was typically performed on day 7. As negative control, human TNF-α (20ng/ml) was used. To immobilize Notch ligands in the culture plates, plates were incubated with a solution of DNER (Extracellular Domain) (human):Fc (human) (rec.) (5μg/ml) or hCD137(4-1BB)-Fc (5μg/ml). Visualized at three different spots.

Please mouse over