DLK1 (human):Fc (human), (recombinant)

Notch ligand



ALX-201-427-C010	10 µg	268.00 USD

ALX-201-427-C050	50 µg	802.00 USD

Product Specification

Alternative Name:	Protein delta homolog 1, Fetal antigen 1, FA1, pG2

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of human DLK1 (aa 1-303) are fused at the C-terminus to the Fc portion of human IgG.

Long Term Storage:	-20°C

Background / Technical Information:	UniProt ID P80370: DLK1 (human)

Figure: SDS-PAGE of Dlk1 (human):Fc (human) (rec.) (Prod. No. ALX-201-427).

Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DLK1 (human):Fc (human), (rec.) (Prod. No. ALX-201-427).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For initiation of differentiation, 3T3L-1 cells were cultured in growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX and 10μg/ml insulin (day 0). The Medium was changed every 2 days and staining with Oil Red O was typically performed on day 7. As negative control, human TNF-α (20ng/ml) was used. To immobilize Notch ligands in the culture plates, plates were incubated with a solution of DLK1 (human):Fc (human), (rec.) (5μg/ml) or hCD137(4-1BB)-Fc (5μg/ml). Visualized at three different spots.

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