DLL3 (human):Fc (human), (recombinant)

Notch ligand



ALX-201-422-C010	10 µg	353.00 USD

ALX-201-422-C050	50 µg	839.00 USD

Product Specification

Alternative Name:	Delta-like protein 3 (human), Delta3

Endotoxin Content:	<0.1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Lyophilized from a 0.2µm-filtered solution in PBS, pH 7.2.

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL3 (aa 1-466) are fused at the C-terminus to the Fc portion of human IgG1.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q9NYJ7: DLL3 (human)

Figure: SDS-PAGE of DLL3 (human):Fc (human) (rec.) (Prod. No. ALX-201-422)

Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DLL3 (human):Fc (human) (rec.) (Prod. No. ALX-201-422).
Method: 3T3L-1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For initiation of differentiation, 3T3L-1 cells were cultured in growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX and 10μg/ml insulin (day 0). The medium was changed every 2 days and staining with Oil Red O was typically performed on day 7. As negative control, human TNF-α (20ng/ml) was used. To immobilize Notch ligands in the culture plates, plates were incubated with a solution of DLL3 (human):Fc (human) (rec.) (5μg/ml) or hCD137(4-1BB)-Fc (5μg/ml).

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