FGF-19 (human), (recombinant) (His-tag)



ALX-201-420-C010	10 µg	194.00 USD

ALX-201-420-C050	50 µg	401.00 USD

Product Specification

Alternative Name:	Fibroblast growth factor 19

Concentration:	0.3mg/ml

Endotoxin Content:	<1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2μm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. The original signal peptide and the mature peptide of human FGF-19 (aa 1-216) are fused at the C-terminus to a His-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID O95750: Fibroblast Growth Factor-19 (human)

Figure 1: SDS-PAGE of FGF-19 (human) (rec.) (His) (Prod. No. ALX-201-420).

Figure 2: Activation of FRS2alpha and ERK by FGF-19 (human) (rec.) (His) (Prod. No. ALX-201-420) in rat hepatoma cells.Method: H4IIE rat hepatoma cells were serum starved for 16h and then stimulated with recombinant hFGF-19-His or FGF-b for 10min respectively. FGF-b (100ng/ml) was used as positive control. The cell lysate was subjected to immunoblotting with antibodies against pFRS2alpha, pERK1/2, total ERK1/2.Lane 1: control (non-treated)Lane 2: hFGF-19-His 30ng/mlLane 3: hFGF-19-His 100ng/mlLane 4: hFGF-19-His 300ng/mlLane 5: hFGF-19-His 1000ng/mlLane 6: FGF-b 100ng/ul

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