DLK1 (mouse):Fc (human), (recombinant)

Figure 2: Adipogenesis inhibition of 3T3L-1 cells by DLK1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416).
Method: 3T3L-1 cells (mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation, 3T3L-1 cells were cultured in adipogenic medium which was growth medium supplemented with 1μM dexamethasone, 0.5mM IBMX, 10μg/ml insulin (day 0). Medium was changed every 2 days. Staining with oil red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, human TNF-α (20ng/ml) was added. Recombinant human DLK1-Fc (5ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 cells. Visualized at three different spots.

Figure 3: Induction of SOX-9 with treatment of DLK1 (mouse):Fc (human) (rec.) (Prod. No. ALX-201-416).
Method: A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of DLK1 (mouse):Fc (human) (rec.) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Sox-9 and anti-mouse Hes-1 or GAPDH.