Jagged-1 (human):Fc (human), (recombinant)

Notch ligand



ALX-201-390-C010	10 µg	194.00 USD

ALX-201-390-C050	50 µg	401.00 USD

Product Specification

Alternative Name:	HJ1, CD339, JAG1

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of human Jagged-1 (HJ1; CD339) (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	The Notch family of proteins are involved in the regulation of key biological processes such as cell fate determination, proliferation, differentiation, and apoptosis. Jagged-1 (HJ1; CD339), a cell surface glycoprotein, is one of several ligands that activate multiple Notch receptors and is involved in mediating Notch signalling. Jagged-1 signalling through Notch has been shown to play a role in hematopoiesis. Jagged-1 also seems to be involved in early and late stages of mammalian cardiovascular development. It has been shown to enhance fibroblast growth factor-induced angiogenesis (in vitro). Mutations in the human Jagged-1 gene cause Alagille syndrome.Jagged-1 binds to Notch 1 receptors and activates Notch 1-dependent signalling pathways. For example, Jagged-1 inhibits T cell activation and is a promising therapeutically beneficial protein for multiple sclerosis or other autoimmune diseases. Additionally Jagged-1 was shown to block osteoclastogenesis in the presence of RANKL and M-CSF.

Background / Technical Information:	UniProt ID P78504: Jagged-1

Figure 1: SDS-PAGE of Jagged-1 (human):Fc (human) (rec.) (Prod. No. ALX-201-390).

Figure 2: Adipogenesis inhibition of 3T3L-1 Cells.
Method: 3T3L1 cells were maintained in DMEM supplement with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1uM, and 10µg/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml of Jagged-1 (human):Fc (human), (rec.) (Prod. No. ALX-201-390) and CD137 (human):Fc (human), (rec.) (Prod. No. ALX-522-031) as a control Fc. Staining with Oil Red O was typically performed on day 7.

Figure 3: Induction of Hes-1 with the treatment of Jagged-1 (human):Fc (human) (rec.) (Prod. No. ALX-201-390).
Method: A mouse preadipocyte cell line, 3T3L1, was stimulated with 5µg/ml of Jagged-1 (human):Fc (human) (rec.) as in indicated time points and each cell lysate was prepared and subjected to Western blot by using anti-mouse Hes1 or GAPDH.

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General Literature References

Endothelial expression of the Notch ligand Jagged1 is required for vascular smooth muscle development: F.A. High, et al.; PNAS 105, 1955 (2008), Abstract;

NOTCH1 regulates osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblast lineage cells: S. Bai, et al.; J. Biol. Chem. 283, 6509 (2008), Abstract; Full Text

Up-regulation of gene related to anergy in lymphocytes is associated with Notch-mediated human T cell suppression: A.M. Kostianovsky, et al.; J. Immunol. 178, 6158 (2007), Abstract; Full Text

Jagged1 protein enhances the differentiation of mesenchymal stem cells into cardiomyocytes: H. Li, et al.; BBRC 341, 320 (2006), Abstract;

Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation: B.W. Purow, et al.; Cancer Res. 65, 2353 (2005), Abstract; Full Text

Mutations in the human Jagged1 gene are responsible for Alagille syndrome: T. Oda, et al.; Nat. Genet. 16, 235 (1997), Abstract;

An antisense oligonucleotide to the notch ligand jagged enhances fibroblast growth factor-induced angiogenesis in vitro: A.B. Zimrin. et al.; J. Biol. Chem. 271, 32499 (1996), Abstract; Full Text