Progranulin (mouse), (recombinant)



ALX-201-389-C010	10 µg	185.00 USD

ALX-201-389-C050	50 µg	405.00 USD

Product Specification

Alternative Name:	Proepithelin, PEPI, PC cell-derived growth factor

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Signal peptide and mature mouse progranulin (aa 1-589) is fused at the C-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	Progranulin (PGRN) has been shown to be a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signalling cascades that control cell cycle progression and cell motility. PGRN has also been linked to tumorigenesis. The function of PGRN in the central nervous system (CNS) is also of interest, because mutations in the PGRN gene have been identified in cases of frontotemporal degeneration (FTD).

Background / Technical Information:	UniProt ID P28798: Granulins (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of Progranulin (mouse) (rec.) (Prod. No. ALX-201-389).

Figure 2: Deglycosylation of Progranulin (mouse) (rec.) (Prod. No. ALX-201-389). Method: To examine deglycosylation of Progranulin (mouse) (rec.) (Prod. No. ALX-201-389), 1 µg of Progranulin (mouse) (rec.) (Prod. No. ALX-201-389) was denatured with 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. After the addition of NP-40 and G7 Reaction Buffer, two-fold dilutions of PNGase F were added and the reaction mix was incubated for 1 or 3 hour at 37°C. Separation of reaction products was visualized by immunoblotting with antibody against mouse progranulin.Lane 1: non-treatedLane 2: PNGase F 1hLane 3: PNGase F 3h

Figure 3: Effects of phospho-ERK1/2 and non phospho-ERK1/2 using Progranulin (mouse) (recombinant) (Prod. No. ALX-201-389) in MCF10A cells. Method: To examine the signal of phosphop44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37°C over 0, 5, 10, 30 min., respectively, by adding the recombinant protein (500ng/ml) to MCF10A human breast epithelial cells, which were maintained with serum starvation for 24 hours. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 5, 10, 30 min., respectively.

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General Literature References

Progranulin: normal function and role in neurodegeneration: J.L. Eriksen & I.R. Mackenzie; J. Neurochem. 104, 287 (2008), (Review), Abstract;

Progranulin in frontotemporal lobar degeneration and neuroinflammation: Z. Ahmed, et al.; J. Neuroinflammation 4, 7 (2007), (Review), Abstract; Full Text

Progranulin (granulin-epithelin precursor, PC-cell-derived growth factor, acrogranin) mediates tissue repair and tumorigenesis: Z. He & A. Bateman; J. Mol. Med. 81, 600 (2003), Abstract;

Progranulin is a mediator of the wound response: Z. He, et al.; Nat. Med. 9, 225 (2003), Abstract;

Granulins, a novel class of peptide from leukocytes: A. Bateman, et al.; BBRC 173, 1161 (1990), Abstract;