CX3CL1 (soluble) (human), (recombinant)



ALX-201-388-C010	10 µg	194.00 USD

ALX-201-388-C050	50 µg	547.00 USD

Product Specification

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Biological Activity:	Chemotaxis effect of soluble recombinant human CX3CL1 (fractalkine) in THP-1 cells (see Figure 2).

Source/Host:	Produced in HEK 293 cells. Signal peptide and extracellular domain of human CX3CL1 (fractalkine) (aa 1-339) is fused at the C-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID P78423: Fractalkine (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of CX3CL1, Soluble (human) (rec.) (Prod. No. ALX-201-388).

Figure 2: Chemotaxis effect of human CX3CL1, Soluble (human) (rec.) (Prod. No. ALX-201-388) in THP-1 cells. Method: Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24-well transwell polystyrene membrane with 8µm pores. 100ng, 500ng, 1’000ng, 2’500ng and 5’000ng of human CX3CL1 were diluted in RPMI 1640 supplemented with 10mg/ml BSA respectively. CX3CL1 samples were added to the lower chamber. THP-1 cells(0.5x10⁶ cells per well), in the same media with CX3CL1, Soluble (human) (rec.) were added to the upper chamber. After 2 hour incubation at 37°C in 5% CO₂ humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from duplicate wells were enumerated on a hemacytometer by light microscopy.

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