ANGPTL7 (human), (recombinant)



ALX-201-368-C050	50 µg	764.00 USD

Product Specification

Alternative Name:	Angiopoietin-like protein 7, Cornea-derived transcript 6 protein

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. Human ANGPTL7 (angiopoietin-like protein 7) (aa 1-346) is fused at the C-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Miscellaneous/General:	Human ANGPTL7 is produced as a secreted glycoprotein with a molecular mass of 45kDa in SDS-PAGE analysis. Human mRNA is expressed in neural tissues, keratoconus cornea, trabecular meshwork, melanotic melanoma and uterus endometrial cancer, while mouse Angptl7 mRNA is expressed in four-cell embryos, synovial fibroblasts, thymus, uterus and testis. ANGPTL7 reduces tumor growth and aberrant blood vessel formation by inducing massive fibrosis in a mouse xenograft model. It was reported that addition of ANGPTL7 results in a further increase in HSC activities. This suggests that ANGPTL7 may activate signal transduction pathways that cannot be activated by SCF, TPO, IGF-2 or FGF-1. Human ANGPTL7, characterized as potent target gene of WNT/ beta-catenin signalling pathway, is a pharmacogenomics target in the fields of oncology and regenerative medicine.

Background / Technical Information:	UniProt link O43827: Angiopoietin-related protein 7 (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of ANGPTL7 (human) (rec.) (Prod. No. ALX-201-368).

Figure 2: ERK phosphorylation is induced by ANGPTL7 (human) (rec.) (Prod. No. ALX-201-368) in THP-1 cells.THP-1 monocyte cells were serum starved for 16h and then stimulated with ANGPTL7 (human) (rec.) for 1,2 and 3h, respectively. The cell lysates were subjected to immunoblotting with antibodies against pERK1/2 or against ERK1/2.

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General Literature References

Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells: C.C. Zhang, et al.; Nat. Med. 12, 240 (2006), Abstract;

Comparative integromics on Angiopoietin family members: Y. Katoh and M. Katoh; Int. J. Mol. Med. 17, 1145 (2006), Abstract;

The angiopoietin-like factor cornea-derived transcript 6 is a putative morphogen for human cornea: R. Peek, et al.; J. Biol. Chem. 277, 686 (2002), Abstract; Full Text