IL-33 (human), (recombinant)



ALX-201-363-C010	10 µg	231.00 USD

ALX-201-363-C050	50 µg	693.00 USD

Product Specification

Alternative Name:	IL-1F11, Interleukin-33, Interleukin 33, NF-HEV

Concentration:	0.5mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL-test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS, pH 7.2.

Biological Activity:	Activates ST2-dependent NF-κB pathway.

Source/Host:	Produced in HEK 293 cells. Recombinant human IL-33 (aa 112-270) is fused at the C-terminus to a FLAG^®-tag.

Specificity:	Binds to human ST2.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q2YEJ5: Interleukin-33 (human) (recombinant) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of IL-33 (human) (rec.) (Prod. No. ALX-201-363).

Figure 2: Activation of a human ST2-dependent NF-κB pathway using IL-33. Method: HEK 293 cells were transiently co-transfected with 500ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-κB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in 12-well plate. At 40 hours after transfection, cells were treated with IL-33 (human) (recombinant) (Prod. No. ALX-201-363) at the concentration indicated for 8 hours, followed by measurement of dual luciferase assay.

Figure 3: Specific binding of IL-33 (human) (rec.) (Prod. No. ALX-201-363) to ST2 (human):Fc (human) (rec.) (Prod. No. ALX-201-367) in vitro.Method: An indirect competitive ELISA was performed as follows; 1) Coat microtiter plate wells with human ST2-Fc (10µg/ml); 2) Add varying concentrations of human IL-33 with or without a human IL-33 MAb to the wells followed by washing; 3) Add anti-FLAG^® HRP conjugated (1:2’000) to an enzyme;4) After adding the TMB solution, incubate at RT in the dark for 10 to 45 min. Immediately read the plate at 450nm.

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