ACE2 (soluble) (human), (recombinant)



ALX-201-356-C010	10 µg	336.00 USD

ALX-201-356-C050	50 µg	799.00 USD

Replaces Prod. #: BML-SE520

Product Specification

Alternative Name:	Angiotensin-converting enzyme 2, SARS receptor

Endotoxin Content:	<0.1EU/µg protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Biological Activity:	Converts angiotensin I to angiotensin-(1-9).

EC:	3.4.17.-

Source/Host:	Produced in HEK 293 cells. The signal peptide sequence and the extracellular domain of human ACE2 (angiotensin-converting enzyme 2) (aa 1-740) are fused at the C-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Miscellaneous/General:	ACE2 is a cell membrane-inserted zinc carboxypeptidase involved in the processing of neuropeptides, possessing 42% sequence identity to ACE1. Drug discovery interests include gastritis and inflammatory bowel disease.

Background / Technical Information:	UniProt ID Q9NRA7: Angiotensin converting enzyme-like protein (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure: SDS-PAGE of ACE2, Soluble (human) (recombinant) (Prod. No. ALX-201-356).

Figure: Activity test using ACE2, Soluble (human) (recombinant) (FLAG^®) (Prod. No. ALX-201-356): Hydrolysis of the fluorescent peptide substrate Mca-YVADAPK(Dnp)-OH.Method: To each test tube, 2ml of assay buffer (75mM TRIS, pH 7.4, 1M sodium chloride, 10µM zinc chloride, 10µM fluorescent peptide substrate) was added. Reactions were initiated by addition of 20µl (1µg/ml, final concentration 10ng/ml) of ACE2, Soluble (human) (recombinant) (FLAG^®), a FLAG tag control protein and buffer only (for blank). Reactions were performed for 20 min. at room temperature and stopped by addition of 0.2ml of 500mM EDTA. Fluorescence intensity was measured using luminescence spectrometer (Ex=320nm, Em=405nm).

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General Literature References

Novel peptide inhibitors of angiotensin-converting enzyme 2: L. Huang, et al.; J. Biol. Chem. 278, 15532 (2003), Abstract;

ACEH/ACE2 is a novel mammalian metallocarboxypeptidase and a homologue of angiotensin-converting enzyme insensitive to ACE inhibitors: A.J. Turner, et al.; Can. J. Physiol. Pharmacol. 80, 346 (2002), Abstract;

A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase: S.R. Tipnis, et al.; J. Biol. Chem. 275, 33238 (2000), Abstract;

A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotensin I to angiotensin 1-9: M. Donoghue, et al.; Circ. Res. 87, E1 (2000), Abstract;