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Nampt (Visfatin/PBEF) (rat), (recombinant) (His-tag)

 
ALX-201-332-C050 50 µg 519.00 USD
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Product Specification

Alternative Name:Pre-B-cell colony enhancing factor 1, Nicotinamide phosphoribosyltransferase
 
Source:Produced in E. coli. Full length rat Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
 
UniProt ID:Q80Z29
 
Formulation:Lyophilized from 0.2μm-filtered solution in 0.1M ammonium bicarbonate, pH 7.2.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<1EU/µg protein (LAL test).
 
Biological Activity:Shows adipogenic effect in differentiating 3T3-L1 cells.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Working aliquots are stable for up to 3 months when stored at -20°C.
 
Handling:For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Nampt (Visfatin/PBEF) (rat), (recombinant) (His-tag) image
Figure 2: Adipogenic effect of Nampt (Visfatin/PBEF) (rat) (rec.) (His) (Prod. No. ALX-201-332) on differentiating 3T3-L1 cells.Method: 5µg/ml recombinant rat Nampt or human insulin was added to the differentiating 3T3-L1 cells that had been stimulated with 1µM dexamethasone and 0.5mM IBMX for 2 days. After 5 days, fat droplets were stained with Oil-Red O.
Nampt (Visfatin/PBEF) (rat), (recombinant) (His-tag) SDS-PAGE
Figure 1: SDS-PAGE of Nampt (Visfatin/PBEF) (rat) (rec.) (His) (Prod. No. ALX-201-332).
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Nampt (Visfatin/PBEF) (rat), (recombinant) (His-tag) image Nampt (Visfatin/PBEF) (rat), (recombinant) (His-tag) SDS-PAGE

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