CD137L (soluble) (mouse), (recombinant)



ALX-201-321-C050	50 µg	405.00 USD

Product Specification

Alternative Name:	4-1BBL, TNFSF 9

Concentration:	1mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL test).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in PBS.

Source/Host:	Produced in HEK 293 cells. The extracellular domain of mouse CD137L (4-1BBL) (aa 104-309) is fused at the N-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID P41274: CD137L (mouse) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of CD137L, Soluble (mouse) (rec.) (Prod. No. ALX-201-321).

Figure 2: CD137L, Soluble (mouse) (recombinant) (Prod. No. ALX-201-321) bound to mouse CD137-transfected cells. HEK-293 cells were transiently transfected with a construct encoding mouse CD137 full length. At 48 hours after transfection, the transfectants were incubated with 0.5 or 5µg/ml of CD137L, Soluble (mouse) (recombinant) and followed by flow cytometric analysis with anti-FLAG M2-FITC. A: Vector only transfectants with 0.5µg/ml of FLAG-mCD137L. B: Vector only transfectants with 5µg/ml of FLAG-mCD137L. C: Mouse CD137 transfectants with 0.5µg/ml of FLAG-mCD137L. D: Mouse CD137 transfectants with 5µg/ml of FLAG-mCD137L.

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