Adiponectin (globular domain) (human), (recombinant)



ALX-201-305-C050	50 µg	382.00 USD

Product Specification

Alternative Name:	Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28

Concentration:	0.4mg/ml

Endotoxin Content:	<0.1EU/µg protein (LAL test; Bio Whittaker).

Purity:	≥90% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in 30mM TRIS, pH 8.5.

Biological Activity:	Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. Activates AMPK in HUVEC cells in a PI(3)K-independent way.

Source/Host:	Produced in HEK 293 cells. The globular domain of human adiponectin (aa 104-244) is fused at the N-terminus to a FLAG^®-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q15848: Adiponectin (human) FLAG is a registered trademark of Sigma-Aldrich Co.

Figure 1: SDS-PAGE of Adiponectin (Globular Domain) (human) (rec.)(Prod. No. ALX-201-305).

Figure 2: Effect of human adiponectin (globular domain) on the phosphorylation of AMPK in HUVEC cells.Method: HUVEC cells were treated with human adiponectin (globular domain) (Prod. No. ALX-210-305). AMPK is activated with a maximal activity at 40 min. This phosphorylation is PI(3)K-independent, as LY294002 (Prod. No. ALX-270-038) and wortmannin (Prod. No. ALX-350-020) cannot inhibit the phosphorylation.

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General Literature References

ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism: A.H. Berg, et al.; Trends Endocrinol. Metab. 13, 84 (2002), (Review), Abstract;