Adiponectin (globular domain) (human), (recombinant) (His-tag)



ALX-201-304-C050	50 µg	382.00 USD

Product Specification

Alternative Name:	Adipocyte complement related protein of 30kDa, ACRP30, AdipoQ, apM1, GBP28

Concentration:	1mg/ml

Endotoxin Content:	<1EU/µg protein (LAL test; Bio Whittaker).

Purity:	≥95% (SDS-PAGE)

Formulation:	Liquid. 0.2µm-filtered solution in 30mM TRIS-Cl, pH 8.5.

Biological Activity:	Effect on phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells (see Fig. 2).

Source/Host:	Produced in E. coli. The globular domain of human adiponectin (aa 104-244) is fused at the C-terminus to a His-tag.

Long Term Storage:	-20°C

Use/Stability:	Working aliquots are stable for up to 3 months when stored at -20°C.

Handling:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Background / Technical Information:	UniProt ID Q15848: Adiponectin (human)

Figure 1: SDS-PAGE using human recombinant His-tagged Adiponectin (Globular Domain) (Prod. No. ALX-201-304).

Figure 2: Effect of human recombinant His-tagged Adiponectin (Globular Domain) (Prod. No. ALX-201-304) on the phosphorylation of acetyl-CoA carboxylase(ACC) in C2C12 cells. C2C12 myocytes were treated with 3μg/ml of the recombinant proteins for 15 min. after serum starvation for 24 hours. 100µg per well of total cell lysate were separated on denatured SDS-PAGE gel. Immunoblot analysis was performed to measure phosphorylation of ACC with anti-phosphoACC antibody.Lane 1: 3μg/ml of human recombinant His-tagged Adiponectin (Globular Domain). Lane 2: 3μg/ml of a His-tagged control protein. Lane 3: Same volume of PBS.

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General Literature References

ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism: A.H. Berg, et al.; Trends Endocrinol. Metab. 13, 84 (2002), (Review), Abstract;