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ARH3 (human), (recombinant) (His-tag)

 
ALX-201-292-C010 10 µg 336.00 USD
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Product Specification

Alternative Name:ADP-ribosylhydrolase 3, ADPRHL2
 
Recommended Dilutions/Conditions:Functional Application (1:10-1:100)
 
Source:Produced in E. coli. Human ARH3 is fused at the C-terminus to a His-tag.
 
EC:3.2.1.143
 
UniProt ID:Q9NX46
 
Concentration:0.2mg/ml
 
Formulation:Liquid. In 20mM HEPES, pH 7.0, containing 140mM KCl, 5mM sodium chloride, 1mM MgCl2 and 50% glycerol.
 
Purity:≥98%
 
Purity Detail:Affinity purified
 
Applications:FUNC
 
Application Notes:Functional Application: Hydrolysation of poly-ADP-ribosylated proteins
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

An HTS-compatible HTRF assay measuring the glycohydrolase activity of human PARG: A.L. Stowell, et al.; Anal. Biochem. 503, 58 (2016), Abstract;
The structure of human ADP-ribosylhydrolase 3 (ARH3) provides insights into the reversibility of protein ADP-ribosylation: C. Mueller-Dieckmann, et al.; PNAS 103, 15026 (2006), Abstract; Full Text

General Literature References

Functional localization of two poly(ADP-ribose)-degrading enzymes to the mitochondrial matrix: M. Niere, et al.; Mol. Cell. Biol. 28, 814 (2008), Abstract; Full Text
Mammalian ADP-ribosyltransferases and ADP-ribosylhydrolases: F. Koch-Nolte, et al.; Front. Biosc. 13, 6716 (2008), Abstract;
Functional aspects of protein mono-ADP-ribosylation: D. Corda & M. Di Girolamo; EMBO J. 22, 1953 (2003), Abstract; Full Text

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Produced in E. coli. Human ARH1 is fused at the C-terminus to a His-tag., ≥98%, FUNC | Print as PDF
 
ALX-201-290-C010 10 µg 336.00 USD
Do you need bulk/larger quantities?
 

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