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NF-κB (p65) (human), (recombinant) (His-tag)

 
ALX-201-284-C002 2 µg 320.00 USD
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Product Specification

Source:Produced in SF21 insect cells. Fused to a His-tag. Produced in a baculovirus expression system.
 
UniProt ID:Q04206
 
Concentration:0.5mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
 
Purity:≥95% (SDS-PAGE)
 
Specific Activity:~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
 

Product Literature References

MYBBP1a is a novel repressor of NF-κB: H. R. Owen, et al.; J. Mol. Biol. 366, 725 (2007), Abstract;
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005), Abstract; Full Text
Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression: M. Covic, et al.; EMBO J. 24, 85 (2005), Abstract; Full Text
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003), Abstract; Full Text

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