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PARP-1 (BRCT domain) (human), (recombinant) (His-tag)

 
ALX-201-255-C020 20 µg 398.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase 1
 
Source:Produced in E. coli. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag.
 
UniProt ID:P09874
 
Concentration:1mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 8, containing 200mM sodium chloride, 60mM imidazole, 0.5mM PMSF and 10% glycerol.
 
Purity:≥99% (SDS-PAGE)
 
Purity Detail:Affinity purified on Ni-beads.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
201-255
Figure: Total cell extract from E. coli BL21 cells before (lane 1) and after (lane 2) induction of the expression of human PARP-1 (BRCT Domain) (human) (recombinant) (His) (Prod. No. ALX-201-255) with 0.5mM IPTG for 2 hours. Lanes 3-5: Purified human PARP-1 (BRCT Domain). 
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201-255

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