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[E988K]PARP-1 (human), (recombinant) (Control)

 
ALX-201-254-C010 10 µg 142.00 USD
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Product Specification

Alternative Name:Poly(ADP-ribose) polymerase 1
 
Source:Produced in Sf21 insect cells. Human full-length inactive mutant E988K of PARP-1 is fused to a His-tag. Produced in a baculovirus expression system.
 
UniProt ID:P09874
 
Concentration:0.5mg/ml
 
Formulation:Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
 
Purity:≥95% (SDS-PAGE)
 
Specific Activity:0.5% of wild type PARP-1.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 

Product Literature References

Mapping PARP-1 auto-ADP-ribosylation sites by liquid chromatography-tandem mass spectrometry: J.D. Chapman, et al.; J. Proteome Res. 12, 1868 (2013), Abstract;
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem. 276, 45588 (2001), Abstract; Full Text

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The PARP Family & ADP Ribosylation
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