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Matriptase (human), (recombinant) (His-tag)

 
ALX-201-246-U250 250 U 448.00 USD
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Product Specification

Alternative Name:MT-SP1, Membrane-type serine protease 1, ST14, Suppression of tumorigenicity protein 14, TADG-15, Tumor-associated differentially expressed gene 15 protein, SNC19
 
MW:~25kDa
 
Source:Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag.
 
UniProt ID:Q9Y5Y6
 
Concentration:100nM
 
Formulation:Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride, 1mM β-mercaptoethanol and 10% glycerol.
 
Purity:≥98% (SDS-PAGE)
 
Activity:Trypsin-like serine protease.
 
Specific Activity:~2.5U/µl. One unit is defined as the amount of enzyme that releases 10pmol of AMC per min. at 37°C in the presence of 50µM of Boc-Gln-Ala-Arg-AMC.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least 6 months when stored at -20°C. Do not store diluted solutions.
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Recommended Assay Buffer: 100mM TRIS, pH 9.5, containing 5mg/ml BSA.
 
ALX-201-246 SDS-PAGE
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ALX-201-246 SDS-PAGE

Product Literature References

Probing the substrate specificities of matriptase, matriptase-2, hepsin and DESC1 with internally quenched fluorescent peptides: F. Beliveau, et al.; FEBS J. 276, 2213 (2009), Abstract;
Inhibition of human matriptase by eglin c variants: A. Désilets, et al.; FEBS Lett. 580, 2227 (2006), Abstract;
Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue: T. Takeuchi, et al.; PNAS 96, 11054 (1999), Abstract; Full Text

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