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Leptin (rat), (recombinant)

 
ALX-201-231-M001 1 mg 236.00 USD
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Product Specification

Alternative Name:OB gene product
 
MW:~16kDa.
 
Source:Produced in E. coli. Homologous to rat serum leptin.
 
MI:14: 5443
 
UniProt ID:P50596
 
Formulation:Lyophilized from 0.0045mM sodium bicarbonate.
 
Purity:≥98% (SDS-PAGE)
 
Endotoxin Content:<0.1EU/µg purified protein.
 
Biological Activity:Induces proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
 
Reconstitution:Reconstitute with sterile water or 0.4% sodium bicarbonate to 0.5-1.0mg/ml, pH 8-9. Never vortex. Do not reconstitute to less than 0.1mg/ml. Further dilutions should be made with medium containing a carrier protein (BSA or HSA 0.1%) and filter sterilized.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Reconstituted protein is stable for 4 weeks when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
 
Scientific Background:Leptin, the product of the ob (obese) gene, is a 16kDa protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.
 

General Literature References

Leptin and the regulation of body weight in mammals: J.M. Friedman and J.L. Halaas; Nature 395, 763 (1998), review, Abstract;
Mutation screening and identification of a sequence variation in the human ob gene coding region: R.V. Considine, et al.; BBRC 220, 735 (1996), Abstract;
Serum immunoreactive leptin concentrations in normal-weight and obese humans  : R.V. Considine, et al. ; New Engl. J. Med. 334, 292 (1996), Abstract;
Effects of the obese gene product on body weight regulation in ob/ob mice  : M.A. Pelleymounter, et al. ; Science 269, 540 (1995), Abstract;
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity  : R.V. Considine, et al. ; J. Clin. Invest 95, 2986 (1995), Abstract;
Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects  : M. Maffei, et al.; Nature Medicine 1, 1155 (1995), Abstract;
Overexpression of the obese (ob) gene in adipose tissue of the human subjects  : F. Lönnqvist, et al. ; Nature Medicine 1, 950 (1995), Abstract;
Positional cloning of the mouse obese gene and its human homologue  : Y. Zhang, et al.; Nature 372, 425 (1994), Abstract;

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