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Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (enriched)

 
ALX-201-180-R050 50 µl 230.00 USD
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Product Specification

Alternative Name:GTP pyrophosphate-lyase (cycling), sGCα1/sGCβ1
 
Source:Produced in Sf9 cells. Fused at the C-terminus to a His-tag.
 
EC:4.6.1.2
 
UniProt ID:Q02108 (α1 subunit), Q02153 (β1 subunit)
 
GenBank ID:AAB94794 (α1 subunit), AAH47620 (β1 subunit)
 
Concentration:≥1.5mg/ml (total protein)
 
Formulation:Liquid. Enriched fraction in 50mM TEA, pH 7.4 containing 1mM EDTA/EGTA, 5mM GSH and 30% glycerol.
 
Purity Detail:Prepared by one column purification.
 
Specific Activity:- Basal: ≥0.001µmol/mg/min.
- 200µM DEA NONOate (Prod. No. ALX-430-034): ≥0.5µmol/mg/min.
- 1µM BAY41-2272 (Prod. No. ALX-420-030): ≥0.01µmol/mg/min.
 
Application Notes:Enzymatic studies and drug screening.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-80°C
 
Use/Stability:We do not recommend to store for longer periods after opening and thawing of the product.
 
Handling:Avoid freeze/thaw cycles.
 

Product Literature References

Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001), Abstract;
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000), Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999), Abstract;
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998), Abstract; Full Text
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981), Abstract;

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