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Guanylyl cyclase α1β1 (soluble) (human), (recombinant) (His-tag) (purified)

 
ALX-201-177-C010 10 µg 349.00 USD
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Product Specification

Alternative Name:GTP pyrophosphate-lyase (cycling), sGCα1/sGCβ1
 
MW:~70kDa/~86kDa / subunit, respectively; heterodimer.
 
Source:Produced in Sf9 insect cells. Fused at the C-terminus to a His-tag. Produced in a baculovirus expression system.
 
EC:4.6.1.2
 
UniProt ID:Q02108 (GCα1), Q02153 (GCβ1)
 
Formulation:Liquid. In 50mM Triethanolamine, pH 7.4, containing 200mM NaCl, 1mM DTT, and 15% glycerol.
 
Purity:≥90% (SDS-PAGE)
 
Specific Activity:Basal: ≥0.050 µmol/mg/min.
- 100µM DEA NONOate (Prod. No. ALX-430-034) ≥10 µmol/mg/min.
 
Application Notes:Biophysical and enzymatic studies and drug screening.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:We do not recommend to store for longer periods after opening and thawing of the product.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Guanylyl cyclase is part of the G protein signaling cascade that is activated by low intracellular calcium levels and inhibited by high intracellular calcium levels. In response to calcium levels, guanylyl cyclase synthesizes cGMP from GTP. cGMP keeps cGMP-gated channels open, allowing for the entry of calcium into the cell.
 
201-177
Figure: Spectral characteristics of basal and NO-treated purified human sGCα1β1 (3µM). Inset – Coomassie staining of 3µg of purified sGCα1β1. PM- protein marker.
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201-177

Product Literature References

Abacavir increases platelet reactivity via competitive inhibition of soluble guanylyl cyclase: P.D. Baum, et al.; AIDS 25, 2243 (2011), Application(s): Assay, Abstract; Full Text
Inhibition of nitric oxide-activated guanylyl cyclase by calmodulin antagonists: L.R. James, et al.; Br. J. Pharmacol. 158, 1454 (2009), Abstract; Full Text
Nitric oxide production from nitrite occurs primarily in tissues not in the blood: critical role of xanthine oxidase and aldehyde oxidase: H. Li, et al.; J. Biol. Chem. 283, 17855 (2008), Abstract; Full Text
Human soluble guanylate cyclase: functional expression, purification and structural characterization: D.N. Kosarikov, et al.; Arch. Biochem. Biophys. 388, 185 (2001), Abstract;
Human recombinant soluble guanylyl cyclase: expression, purification, and regulation: Y.C. Lee, et al.; PNAS 97, 10763 (2000), Abstract; Full Text
Purified soluble guanylyl cyclase expressed in a baculovirus/Sf9 system: stimulation by YC-1, nitric oxide, and carbon monoxide: M. Hoenicka, et al.; J. Mol. Med. 77, 14 (1999), Abstract;
Human soluble guanylate cyclase: functional expression and revised isoenzyme family: U. Zabel, et al.; Biochem. J. 335 (Pt 1), 51 (1998), Abstract; Full Text
Soluble guanylate cyclase purified from bovine lung contains heme and copper: R. Gerzer, et al.; FEBS Lett. 132, 71 (1981), Abstract;

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