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Caspase-8 (mouse), (recombinant) (active)

ALX-201-163-C020 20 µg 328.00 USD
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Product Specification

Alternative Name:FLICE, Mch5, MACH
Source:Produced in E. coli.
UniProt ID:O89110
Formulation:Liquid. In 50mM HEPES, pH 7.4, containing 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 50% glycerol.
Purity:≥90% (SDS-PAGE)
Specific Activity:≥100U/µg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate Z-IETD-AMC (AMC: 7-aminomethylcoumarin) (Prod. No. ALX-260-042) per hour at +30°C in a reaction solution containing 20µM Z-IETD-AMC, 50mM HEPES, pH 7.4, 100mM NaCl, 0.5% CHAPS, 10mM DTT, 1mM EDTA and 10% glycerol.
Shipping:Shipped on Dry Ice
Short Term Storage:-20°C
Long Term Storage:-80°C
Use/Stability:Stability at -20°C: 1-2 weeks.
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Technical Info/Product Notes:Note: Activity of this enzyme may essentially decrease after repeated freezing and thawing. For consistent results, it is recommended to use fresh aliquots.

Product Literature References

Identification and characterization of a novel mammalian caspase with proapoptotic activity: L. Eckhart, et al.; J. Biol. Chem. 280, 35077 (2005), Abstract; Full Text

General Literature References

Tumor Necrosis Factor-induced Toxic Liver Injury Results from JNK2-dependent Activation of Caspase-8 and the Mitochondrial Death Pathway: Y. Wang, et al.; J. Biol. Chem. 281, 15258 (2006), Abstract;

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