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Caspase-9 (human), (recombinant) (active)

 
ALX-201-136-C005 5 µg 314.00 USD
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Product Specification

Alternative Name:ICE-LAP-6, Mch6
 
Source:Produced in E. coli.
 
EC:3.4.22.-
 
UniProt ID:P55211
 
Concentration:0.2mg/ml
 
Formulation:Liquid. In 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for at least one week when stored at +4°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:When expressed in E. coli, caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme.
The rate of caspase-9 enzymatic activity can be measured by the release of AMC from the caspase substrate Ac-LEHD-AMC (Prod. No. ALX-260-080) as emission at 440nm and excitation at 380nm using a spectrofluorometer.
 

Product Literature References

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997), Abstract; Full Text

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Ac-LEHD-AMC 

Caspase-9 substrate
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ALX-260-080-M001 1 mg 51.00 USD
 
ALX-260-080-M005 5 mg 144.00 USD
Do you need bulk/larger quantities?
 

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For Research Use Only. Not for use in diagnostic procedures.
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