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CD14 (mouse), (recombinant) (His-tag)

 
ALX-201-134-C010 10 µg 448.00 USD
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Product Specification

MW:~45kDa
 
Source:Produced in CHO cells.
 
UniProt ID:P10810
 
Concentration:0.25mg/ml after reconstitution.
 
Formulation:Lyophilized from PBS, pH 7.2.
 
Purity:≥90-95% (SDS-PAGE)
 
Purity Detail:His-tag-affinity purified.
 
Endotoxin Content:<0.00015EU/µg protein (LAL test; Charles River).
 
Biological Activity:Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS).
 
Reconstitution:Reconstitute with 40µl sterile, ultra-pure distilled water. This solution can then be diluted with PBS or other buffers.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:The reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA).
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
 

Product Literature References

Absence of TRIF signaling in lipopolysaccharide-stimulated murine mast cells: S. Keck, et al.; J. Immunol. 186, 5478 (2011), Abstract;
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996), Abstract;

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