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CD14 (human), (recombinant)

 
ALX-201-133-C010 10 µg 351.00 USD
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Product Specification

MW:~50kDa.
 
Source:Produced in CHO cells.
 
UniProt ID:P08571
 
Concentration:0.25mg/ml after reconstitution.
 
Formulation:Lyophilized from PBS, pH 7.2.
 
Purity:≥90-95% (SDS-PAGE)
 
Purity Detail:Affinity purified using MAb to CD14 (human) (biG 2/RoMo-1) (Prod. No. ALX-804-498).
 
Endotoxin Content:<1EU/ml (LAL-test Charles River).
 
Biological Activity:Inhibits binding of LPS to CD14. Up to 20µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6x105 CD14+ CHO transfectants (FACS).
 
Reconstitution:Reconstitute with 40µl distilled water. Further dilutions should be made with PBS.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore it is present in a soluble form in mouse serum, urine and other body fluids.
 
201-133-better
Figure: SDS-PAGE of CD14 (human) (rec.) (Prod. No. ALX-201-133).
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201-133-better

Product Literature References

Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation: K. Le-Barillec, et al.; J. Clin. Invest. 103, 1039 (1999), Abstract; Full Text
The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms: F. Stelter, et al.; Eur. J. Biochem. 236, 457 (1996), Abstract;
Both membrane-bound and soluble forms of CD14 bind to gram-negative bacteria: R.S. Jack, et al.; Eur. J. Immunol. 25, 1436 (1995), Abstract;

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