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LBP (human), (recombinant) (His-tag)

 
ALX-201-131-C010 10 µg 371.00 USD
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Product Specification

Alternative Name:Lipopolysaccharide binding protein
 
Source:Produced in CHO cells.
 
UniProt ID:P18428
 
Concentration:0.25mg/ml after reconstitution.
 
Formulation:Lyophilized. Contains PBS, pH 7.2.
 
Purity:≥90-95% (SDS-PAGE)
 
Endotoxin Content:<0.00015EU/µg protein (LAL test; Charles River).
 
Biological Activity:Mediates binding of LPS to CD14 (up to 0.2µg/ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+ CHO transfectants (FACS)).
 
Reconstitution:Reconstitute with 40µl distilled water.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-80°C
 
Use/Stability:Will precipitate by rapid changes of pH or temperature. Best working temperature is ice bath.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:LBP (lipopolysaccharide binding protein) is a ~58kDa acute phase glycoprotein and binds to the lipid A portion of lipopolysaccharide (LPS) with high affinity and catalyzes the CD14 dependent cellular activation by LPS.
 
Technical Info/Product Notes:Note: His-tag at the C-terminal end of the LBP has no protease site and cannot be split off.
 
201-131
Figure: SDS-PAGE of LBP (human) (rec.) (His) (Prod. No. ALX-201-131).
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201-131

Product Literature References

Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996), Abstract;

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