Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

SuperKillerTRAIL™ (soluble) (mouse), (recombinant)

 
ALX-201-130-C020 20 µg 412.00 USD
 
ALX-201-130-3020 3x20 µg 895.00 USD
Do you need bulk/larger quantities?
 
SuperKillerTRAIL™ (soluble) (mouse), (recombinant) is an enhanced ligand that utilizes the Killer™ linker peptide mutated to increase disulfide-mediated cross-linking to form a more stable oligomer.

It has been well established that signals transmitted via TNF ligands and receptors are critical in mediating inflammation, cancer, and immunity. This has led to drug development aiming to either reduce or enhance receptor response. One limitation in this process is the inherent instability of recombinant ligands and their inability to effectively mimic the natural clustering of TNF receptors on the cell surface required for immune activation. Oligomerization of TNF ligands using enhancers such as SuperKillerTRAIL™ has been shown to improve stability and significantly enhance immune activation compared to recombinant ligands alone.

Product Specification

Alternative Name:TNFSF 10, Apo-2L, CD253
 
Source:Produced in E. coli. The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
 
UniProt ID:P50591
 
Gene/Protein Identifier:A002862 (UCSD Signaling Gateway ID)
 
Concentration:0.5mg/ml.
 
Formulation:Liquid. In 20mM HEPES, pH 7.4, 300mM sodium chloride, 0.01% Tween 20, 1% sucrose, 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Affinity purified. Sterile filtered.
 
Endotoxin Content:<10.0EU/mg purified protein (LAL test; Bio Whittaker).
 
Specificity:Binds to mouse and less potently to human TRAIL receptors.
 
Biological Activity:Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors (e.g. Prod. No. ALX-522-067 (rmTRAIL-R2:Fc)).
Note: This protein does not require a cross-linker (enhancer) for its potent biological activity.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C. For a special storage and dilution buffer see Prod. No. ALX-505-005.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:TNF-related apoptosis-inducing ligand (TRAIL) is a protein ligand that induces the process of cell death called apoptosis.
 

Please mouse over

Product Literature References

Tumor necrosis factor-related apoptosis-inducing ligand mediates the resolution of allergic airway inflammation induced by chronic allergen inhalation: L. Faustino, et al.; Mucosal Immunol. 7, 1199 (2014), Abstract;
Loss of Caspase-9 Provides Genetic Evidence for the Type I/II Concept of CD95-mediated Apoptosis: A.K. Samraj, et al.; J. Biol. Chem. 281, 29652 (2006), Application(s): Death induction of J16, JMR, and F9 cells, Abstract; Full Text

Related Products

KillerTRAIL™ Storage and Dilution Buffer 

Print as PDF
 
ALX-505-005-R500 500 µl 170.00 USD
Do you need bulk/larger quantities?
 

TRAIL-R2 (mouse):Fc (human), (recombinant) 

Produced in HEK 293 cells. The extracellular domain of mouse TRAIL-R2 (DR5) (aa 1-166) is fused to the Fc portion of human IgG1., ≥90% (SDS-PAGE), Flow Cytometry | Print as PDF
 
ALX-522-067-C050 50 µg 419.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
TRAIL
By biological activity:
TRAIL Recombinant protein
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Product Flyers
TRAIL & TRAIL Receptors
TRAIL & TRAIL Receptors
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,